transposase reaction mix (Illumina Inc)

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Illumina Inc transposase reaction mix
Transposase Reaction Mix, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 97/100, based on 990 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tagmentation+reaction/Illumina+Tagment+DNA+Enzyme+and+Buffer+Small+Kit/bio_rxiv__64898__2026__02__18__706382-174-7-10
Average 97 stars, based on 990 article reviews

transposase reaction mix - by Bioz Stars, 2026-10

97/100 stars

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Article Title: Neuroligin-3 interaction with CSPG4 regulates normal and malignant glial precursors through PIEZO1
Article Snippet: Samples were normalized to 0.2 ng/uL and a total of 0.8 ng of cDNA was used as input for tagmentation reaction according to manufacturer’s instructions (Illumina, FC-131- 1096).

Purification:

Article Title: Mouse genome rewriting and tailoring of three important disease loci.
Article Snippet: .. Tn5 transposase was used for the tagmentation reaction (Illumina, 20034197) at 37 °C for 30 min. Fragmented DNAs were purified using a cleanup column (Zymo Research, D4013) and eluted in 10 μl water. ..

Article Title: MYCN mediates cysteine addiction and sensitizes neuroblastoma to ferroptosis
Article Snippet: .. The tagmentation reaction (Illumina Nextera DNA library Prep Kit) was performed at 37 °C for 1 min with the bead-bound chromatin sample or 5 ng purified input DNA for normalization. .. After de-cross-linking, purified samples were amplified using the dual index barcodes of the Nextera Index Kit and 13 PCR cycles.

Article Title: MYCN mediates cysteine addiction and sensitizes to ferroptosis
Article Snippet: Formaldehyde cross-linking, cell lysis, sonication and chromatin immunoprecipitation were performed as previously described , adding the Chipmentation module by Schmidl et al. with following changes: a Bioruptor Plus with automated cooling (4°C) was used for high intensity sonication (20-30 min each with 30 sec on and 30 sec off intervals), and 10 μg MYCN antibody (MYCN (sc-53993, Santa Cruz) and 10 6 cells for chromatin immunoprecipitation. .. The tagmentation reaction (Illumina Nextera DNA library Prep Kit) was performed at 37°C for 1 min with the bead-bound chromatin sample or 5 ng purified input DNA for normalization. .. After de-crosslinking, purified samples were amplified using dual index barcodes of the Nextera Index kit and 13 PCR cycles.

Article Title: Mouse genome rewriting and tailoring of three important disease loci
Article Snippet: .. Tn5 transposase was used for the tagmentation reaction (Illumina, 20034197) at 37 °C for 30 min. Fragmented DNAs were purified using a cleanup column (Zymo Research, D4013) and eluted in 10 μl water. ..

Article Title: MYCN mediates cysteine addiction and sensitizes neuroblastoma to ferroptosis.
Article Snippet: .. The tagmentation reaction (Illumina Nextera DNA library Prep Kit) was performed at 37 °C for 1 min with the bead-bound chromatin sample or 5 ng purified input DNA for normalization. .. After de-cross-linking, purified samples were amplified using the dual index barcodes of the Nextera Index Kit and 13 PCR cycles.

Incubation:

Article Title: A survey of hypothalamic phenotypes identifies molecular and behavioral consequences of MYT1L haploinsufficiency in male and female mice
Article Snippet: .. 100,000 nuclei were added to the tagmentation reaction (Illumina Tagment DNA TDE1 Enzyme and Buffer Kit, 30 minute incubation at 37°C) for each sample. ..

Sequencing:

Article Title: Deletion of a Hand1 lncRNA-Containing Septum Transversum Enhancer Alters lncRNA Expression but Is Not Required for Hand1 Expression
Article Snippet: .. Nuclei were pelleted and 50 μL tagmentation reaction and subsequent sequencing carried out as per manufacturer’s recommendations (Illumina, San Diego, CA, USA). ..

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